How does ammonium fluoride affect chromatography using Cogent TYPE-C HPLC columns?

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ARTICLES, APPNOTES, FAQ (9289)
- AppNotes Library (1888)
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- Frequently Asked Questions?? (3402)
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October 24, 2016
Bill Ciccone

ARTICLES, APPNOTES, FAQ / HPLC Tips and Suggestions / Mobile Phase Suggestions / TYPE-C Column Information

Ammonium fluoride as mobile phase additive functions beneficially in several ways in a for LCMS:

—• As a buffer (i.e. pH control)
—• Improvement of peak shapes
—• Improvement of sensitivity in MS for certain compounds

For further reading, please refer to the following third-party journal article reference:
J. Pesek, M. Matyska, “Ammonium fluoride as a mobile phase additive in aqueous normal phase chromatography,” J. Chromatogr. A. 1401, 2015, 69–74.

NOTE: care should be taken when using ammonium fluoride because it is corrosive to glass (Teflon bottles are preferred) and using a concentration in excess of 1 mM may permanently damage HPLC columns. However, it has been reported that Cogent TYPE-C™ Silica columns last much longer than ordinary columns but ammonium fluoride may somewhat decrease the column lifetime.

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