This graph is from a study investigating the day to day retention time precision with two test solutes on the Cogent Diamond Hydride 2.o™ HPLC column

Blue Trace: Phenylalanine
Red Trace: Phenylglycine

Many factors can affect column robustness and lifetime, so it is not possible to define specific values for those parameters.

Mobile Phase:
A: DI Water / 0.1% Formic Acid
B: Acetonitrile / 0.1% Formic Acid
Flow Rate: 0.3mL / minute
Injection Volume: 0.2uL
Detection: UV 254nm


Time (minutes) %B
0 90
6 20
7 20
8 90


Cogent Diamond Hydride HPLC Column Ordering Information