Peak shape for polar compounds analysis such as vitamins in LCMS has been shown to be improved when using a 0.1% formic acid additive instead of ammonium acetate.
The sample diluent should be 50% DI water/ 50% acetonitrile/ 0.1% formic acid. If you are using 50% isopropanol or 50% methanol in the aqueous solvent, the carryover could be due to insufficient eluting strength.
Try using DI water/ 0.1% formic acid instead of 50% 2-propanol/ 50% DI water/ 0.1% formic acid.