Which HPLC column do you suggest for analysis of carotene or lycopene – FAQ

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ARTICLES, APPNOTES, FAQ (9292)
- AppNotes Library (1888)
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September 18, 2015
Bill Ciccone

ARTICLES, APPNOTES, FAQ / Cogent RP Column Information / Cogent UDC-Cholesterol Information / HPLC Column Information / HPLC Columns FAQ / HPLC Suggestions and Information / Trouble Shooting Suggestions / TYPE-C Column Information / UDC-Cholesterol FAQ

The best option would be either the Cogent C30™ or the Cogent UDC-Cholesterol™

With these types of compounds, the main challenge will be separating the different isomers. There are numerous double bonds in the molecules, and many combinations of cis/trans isomers are likely to be encountered. Because the isomers differ little in hydrophobicity but significantly in shape, a column with shape-selective capabilities would be required for an adequate separation.

Reversed phase selectivity alone, such as from a C8 phase, is generally insufficient. One column with shape selective behavior is the Cogent C30™. The C30 chains will become more rigid at lower temperatures, and therefore selectivity of isomers is predicted to increase at decreasing temperature.

The same principle will apply for the Cogent UDC-Cholesterol™, which also adopts a more rigid conformation at lower temperatures. Hence, temperature will be an important parameter to investigate in method development and the column should always be thermostatted to ensure robustness.

Cogent C30 HPLC Column Ordering Information
Cogent UDC-Cholesterol HPLC Column Ordering Information

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1) Shape and Size Selectivity of HPLC Columns – AppNote

2) Types of isomers and suitable HPLC columns for separation – Tips & Suggestions

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