To obtain greater sensitivity than Na adducts for sugars could you add ammonia NH4 instead – FAQ

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ARTICLES, APPNOTES, FAQ (9289)
- AppNotes Library (1888)
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- Frequently Asked Questions?? (3402)
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April 10, 2015
Bill Ciccone

ARTICLES, APPNOTES, FAQ / Frequently Asked Questions?? / HPLC Suggestions and Information / HPLC Tips and Suggestions / Mobile Phase FAQ / Mobile Phase Suggestions / Trouble Shooting Suggestions

In LCMS, you will get much better sensitivity of Na-sugar adducts in the EICs compared to sugars themselves.

The same principle will work for NH4 adducts as well, but there are notable drawbacks. The problems with NH4 is that it will change the pH of the mobile phase and its concentration is hard to control in the sample. Hence, given both strategies will work, there is no reason to choose ammonia NH4 over sodium Na.

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