If your HPLC pump produces a “pulse” (i.e. cyclic fluctuation in flow/pressure), this can cause problems for your Method and Column. The pump will not be delivering optimally Precise/Accurate Flow and this can cause your system to not meet System Suitability Criteria as well as other issues.
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In order to verify the Precision/Accuracy of your Pump, you can use our HSQ™ Kit to Qualify and be sure these Parameters as well as many others are correct and what you expect.