If your HPLC pump produces a “pulse” (i.e. cyclic fluctuation in flow/pressure), this can cause problems for your Method and Column. The pump will not be delivering optimally Precise/Accurate Flow and this can cause your system to not meet System Suitability Criteria as well as other issues.
Sudden changes in the Pressure can also cause Disruption of the packed bed of the Column, adversely affecting Column Life. We suggest that you use a Pulse Damper in your HPLC System for this reason. One example is a dual piston pump where the two pistons are out of phase. The resulting flow/pressure is more constant.
In order to verify the Precision/Accuracy of your Pump, you can use our HSQ™ Kit to Qualify and be sure these Parameters as well as many others are correct and what you expect.