Can I use the UV-vis Calibration Kit to qualify detection at 200nm – FAQ

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ARTICLES, APPNOTES, FAQ (9292)
- AppNotes Library (1888)
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October 15, 2020
Bill Ciccone

ARTICLES, APPNOTES, FAQ / Frequently Asked Questions?? / How To... Information / HPLC Suggestions and Information / HPLC Tips and Suggestions / Qualification FAQ / Qualification Information / Qualification Tech Information / UV-vis Qualification

The caffeine standard in the kit provides two data points at 205nm and 273nm. The holmium oxide solution in the kit covers 14 absorbance bands in the range of 241nm – 641nm. Hence, you can obtain a calibration curve from these various data points and then extrapolate down to 200nm.

This is the lowest you would need to go because of the UV cutoff of most typical HPLC solvents is just below this.

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