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ARTICLES, APPNOTES, FAQ (9289)
- AppNotes Library (1888)
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- Frequently Asked Questions?? (3402)
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How do retention and efficiency of asymmetric dimethylarginine ADMA compare using 4um vs 2.o Diamond Hydride columns – AppNote

KB Home/ARTICLES, APPNOTES, FAQ / Frequently Asked Questions?? / HPLC Columns FAQ / 2.o™ HPLC Columns FAQ / ARTICLES, APPNOTES, FAQ /ARTICLES, APPNOTES, FAQ / Frequently Asked Questions?? / HPLC Columns FAQ / Diamond Hydride FAQ / How do retention and efficiency of asymmetric dimethylarginine ADMA compare using 4um vs 2.o Diamond Hydride columns – AppNote

May 29, 2013
Bill Ciccone

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2.o™ HPLC Columns FAQ / ARTICLES, APPNOTES, FAQ / Diamond Hydride FAQ

ADMA can be retained using an ANP gradient method with the Cogent Diamond Hydride™ HPLC column.

Retention times for ADMA differed only slightly between the 4um and 2.o™ stationary phases. Efficiency however was notably higher when using the 2.o™ phase. This can be readily observed from the greater peak height using the 2.o™ column in the chromatogram overlay comparison shown below:

Method Conditions:
Column dimensions: 2.1 x 50mm (both)
Flow rate: 0.3mL/min
A: DI H₂O + 0.1% formic acid
B: Acetonitrile + 0.1% formic acid

time (min)    %B
0                90
5                  30
8                  30
10                90
post time 4 min

For more information: Cogent Diamond Hydride Product Page

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