Establishing HPLC method linearity – Tips & Suggestions

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ARTICLES, APPNOTES, FAQ (9289)
- AppNotes Library (1888)
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- Frequently Asked Questions?? (3402)
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May 14, 2013
Bill Ciccone

ARTICLES, APPNOTES, FAQ / Frequently Asked Questions?? / How To... Information / HPLC Primer / HPLC Suggestions and Information / HPLC Tips and Suggestions

To establish linearity of the proposed method, ten point calibration curves should be generated with appropriate concentrations of calibration standard solutions. The calibration range can be for example: 10-100 microgram / mL.

The linearity should be evaluated by the least squares regression method using unweight data.

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