What is the purpose of using isopropanol IPA as part of the mobile phase solvent in ANP methods – FAQ

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ARTICLES, APPNOTES, FAQ (9292)
- AppNotes Library (1888)
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March 11, 2013
Bill Ciccone

ANP Aqueous Normal Phase Articles / ARTICLES, APPNOTES, FAQ / Cogent Diamond Hydride Information / Cogent Phenyl Hydride Information / Frequently Asked Questions?? / HPLC Column Information / HPLC Columns FAQ / HPLC Suggestions and Information / HPLC Tips and Suggestions / Mobile Phase FAQ / Mobile Phase Suggestions / Trouble Shooting Suggestions / TYPE-C Column Information / TYPE-C in General FAQ

Some of our Cogent TYPE-C HPLC column application notes that feature Aqueous Normal Phase ANP use 50% isopropanol (IPA) as part of the aqueous component of the mobile phase. The main reason for this use is to help keep the column free of undesired adsorbed contaminants from a sample or sample matrix.

Contaminants can change the retention behavior of your analyte in any HPLC method and can also slowly elute off the column as carryover peaks in subsequent runs or as baseline noise and drift. We have found IPA (or methanol can be used) to be good at washing these contaminants off the column during the final part of an ANP gradient run. When performing HPLC or LCMS, adding IPA to the A (aqueous) solvent component will have little effect on the chromatography compared to using only aqueous-containing solvents, so it is something you should consider adding in the method before it has been fully developed.

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