How can I separate methacrylic acid and dodecyl benzene sulfonic acid – FAQ

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ARTICLES, APPNOTES, FAQ (9292)
- AppNotes Library (1888)
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December 17, 2012
Bill Ciccone

ARTICLES, APPNOTES, FAQ / Bidentate C18 FAQ / Cogent Bidentate C18 Information / Cogent Phenyl Hydride Information / Diamond Hydride FAQ / How To... Information / HPLC Column Information / HPLC Columns FAQ / HPLC Tips and Suggestions / Phenyl Hydride FAQ / Trouble Shooting Suggestions / TYPE-C Column Information

Good retention and peak shapes have been observed for similar compounds.

Methacrylic acid is similar in structure to acrylic acid, which can be well-retained with a mobile phase of DI water + 0.1% formic acid and a Bidentate C18 column.

Dodecyl benzene sulfonic acid has some of the same functional groups as docusate sodium, which also has a sulfonate group. The latter was observed to elute with a good peak shape using a gradient method and the Cogent Bidentate C18™ column. Dodecyl benzene sulfonic acid by itself should be suitable for the Cogent Diamond Hydride column as well, using an ammonium acetate-based mobile phase.

A gradient starting at high water (to retain the methacrylic acid) and ending at significant organic (to elute the dodecyl benzene sulfonic acid) can be used to retain both compounds. Since a Cogent Bidentate C18™ column was used for the two compounds above, it should be suitable for a separation of methacrylic acid and dodecyl benzene sulfonic acid as well. The Cogent Phenyl Hydride™ column would also be suitable.

Cogent Bidentate C18 ordering information
Cogent Diamond Hydride ordering information
Cogent Phenyl Hydride ordering information

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