Separation of Pyrilamine with a Simple Isocratic Method

Pyrilamine is a challenging compound to analyze with HPLC because it contains several amine groups that can contribute to peak tailing. In this Method, an excellent Peak shape is obtained for Pyrilamine with a simple isocratic Mobile Phase. Data is shown for two lots of Columns in order to demonstrate the robustness and precision of this Method.

The Method is rapid, robust, and suitable for routine Assay of Pyrilamine formulations. The data shown here used a USP reference standard of Pyrilamine Maleate but the conditions can be applied to a variety of formulation extracts.


Method Conditions
Column: Cogent Diamond Hydride™, 4µm, 100Å
Catalog No.: 70000-7.5P
Dimensions: 4.6 x 75mm
Mobile Phase: 8% DI Water / 92% Acetonitrile / 0.1% Trifluoroacetic Acid (TFA) v/v
Injection vol.: 2µL
Flow rate: 1.0mL / minute
Detection: UV @ 244nm
Sample Preparation: 100mg / L Pyrilamine Maleate in diluent of 50:50:0.1 DI Water (v/v) / Acetonitrile/ Trifluoroacetic Acid (TFA)
t0: 0.9 minutes

Note: Pyrilamine (also known as Mepyramine) is a first generation antihistamine. It is found in many common over-the-counter oral and topical formulations with a variety of uses.


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