Doxapram HCL Analyzed with HPLC – AppNote – MicroSolv Technology Corporation

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ARTICLES, APPNOTES, FAQ (9292)
- AppNotes Library (1888)
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August 20, 2012
Bill Ciccone

AppNotes ANP/HILIC Methods / AppNotes Cogent Diamond Hydride / AppNotes Library / AppNotes Pharmaceuticals / ARTICLES, APPNOTES, FAQ

Easy, Rapid Isocratic Assay of A Polar Compound

Automating a Method is a distinct advantage over the official USP Method for Doxapram which is by Titration. Not only will this Method save time and money, but will reduce errors. Simple to perform, the Mobile Phase can be quickly prepared without errors.

A highly Symmetrical Peak is obtained for the analyte leading to reliable Quantitative Analysis. Injections and Chromatograms using two different lots of Columns illustrate the Reproducibility and Robustness of the Method.

Peak:
Doxapram

Method Conditions
Column: Cogent Diamond Hydride™, 4μm, 100Å
Catalog No.: 70000-7.5P
Dimensions: 4.6 x 75mm
Mobile Phase: 5:95 DI Water / Acetonitrile with 0.1% Trifluoroacetic Acid (TFA) v/v
Injection vol.: 1μL
Flow rate: 1.0mL / minute
Detection: UV @ 258nm
Sample Preparation: 1mg / mL Doxapram HCL in diluent of 50:50 DI Water / Acetonitrile with 0.1% Trifluoroacetic Acid (TFA) v/v
t0: 0.9 minutes

Note: Doxapram is a respiratory stimulant. It acts by stimulating chemoreceptors in the carotid bodies of the carotid arteries. It is sold in trade names such as Dopram®, Stimulex®, and Respiram®.

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