Analysis of Amino-Caproic Acid by a Simple LCMS Method

6-Amino-N-Caproic Acid is an active pharmaceutical ingredient used in massive thrombolysis and proteolysis secondary to metastatic carcinoma of the prostate. It is also a potent in vitro inhibitor of fibrinolysis. After oral administration 6-Amino-N-Caproic Acid enhances the uptake of labeled Fibrinogen in both the Walker and Murphy tumors.

The drug is also used in treatment of ulcers and many other diseases. Chromatograms presented (A and B) show the retention of this basic molecule using a simple Isocratic LCMS Method. Observe that when the concentration of Acetonitrile increases the retention of 6-Amino-N-Caproic Acid also increases.

 

Peaks:
1. Uracil m/z 112
2. 6-Amino-N-Caproic Acid m/z 132

Method Conditions
Column: Cogent Bidentate C18™, 4µm, 100Å
Catalog No.: 40018-75P
Dimensions: 4.6 x 75mm
Mobile Phase:
A: 30% DI Water / 70% Acetonitrile / 0.1% Formic Acid
B: 20% DI Water / 80% Acetonitrile / 0.1% Formic Acid
Injection vol.: 2µL
Flow rate: 0.5mL / minute
Detection: Mass Spectrometer – Atmospheric Pressure Chemical Ionization in positive mode: APCI+ Single Ion Monitoring

Note: Mobile phase with 0.1% Formic Acid can be used in the UV detection of 6-Amino-N-Caproic Acid at 206nm.

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