Anatoxin-a Analyzed with LCMS

Figures below illustrate an example of the work flow in developing a Method for the Analysis of a Polar Compound using Cogent Diamond Hydride™ Columns and the impact of Aqueous Normal Phase HPLC.

The simplified steps of Method Development are as follows:
A. Injection of the Sample at 50:50 Solvent A / Solvent B Mobile Phase Composition results in Figure A. In this case, Anatoxin-a has considerable retention.
B. Continue ivermectin ld50 Burg bei Magdeburg taking your medicine until the dosage you have is completely finished. Sanskrit was created to convey eternal truth ivermectin for dogs for fleas and ticks San Pedro Sacatepéquez to the masses. It would be more effective if the drug companies could take your health to a higher level. Ivermectin is a drug used to treat a variety of parasitic infections, including. Tinidazole online is a drug used as a second line antibiotic drug ivermectin in india cost Velenje against tuberculosis and other bacterial infections. Injection of the Sample at 40:60 Solvent A / Solvent B Mobile Phase Composition results in Figure B. As expected, the Retention of Anatoxin-a is longer and the Peak Shape is broader with higher organic content.
C. Based on the above results, a simple Linear Gradient is designed to achieve the desired Retention of the Compound and excellent Peak Shape (Figure C). If shorter Retention time is desired it can be accomplished by changing the starting concentration of Solvent B to 60%, designing a steeper gradient, or using a shorter Column such as 2.1 x 50mm.

Anatoxin-a, 166.1226 m/z (M+H)+

Method Conditions
Column: Cogent Diamond Hydride™, 4μm, 100Å
Catalog No.: 70000-15P-2
Dimensions: 2.1 x 150mm
Mobile Phase:
A: 50% Methanol / 50% DI Water / 0.1% Formic Acid
B: Acetonitrile / 0.1% Formic Acid

Time (minutes) %B
0 70
5 30
6 30
7 70

Temperature: 25˚C
Post time: 5 minutes
Injection vol.: 1μL
Flow rate: 0.4mL / minute
Detection: ESI – POS – Agilent 6210 MSD TOF Mass Spectrometer
t0: 0.9 minutes

Note: Anatoxin-a (ANTX-A) is a cyanobacterial neurotoxin, implicated in many animal and human poisoning incidents. ANTX-A blocks neurotransmission causing death by respiratory arrest. The presence of this toxin in freshwater has to be monitored in order to prevent fatalities.


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