Separating UDP and CDP Sugars with ANP and Increased Sensitivity
This Method can be used to analyze UDP and CDP Sugars using UDP-Hexanolamine (a metabolite) as an Internal standard. The Sugar Nucleotides used in this Application Note are a mixture of compounds that occur in plants and their structure is proprietary.
A potentially powerful tool for profiling Sugar Nucleotides in Metabolomic studies, this Method uses an Inverse Gradient (HILIC like); the Mobile Phase uses high organic component which enhances Mass Spec response and assures lower detection limits.
Column: Cogent Diamond Hydride™, 4μm, 100Å
Catalog No.: 70000-15P-2
Dimensions: 2.1 x 150mm
—A: DI Water / 0.1% Ammonium Formate (pH 7.2)
—B: 90% Acetonitrile / 10% DI Water / 0.1% Ammonium Formate (pH 6)
Post Time: 5 minutes
Flow rate: 0.3mL / minute
Detection: ESI – neg – Agilent 6410 Triple Quadrupole Mass Spectrometer
—1. Compound 1 – the monitored MRM transitions were m/z 535 to m/z 323
—2. Compound 2 – the monitored MRM transitions were m/z 564 to m/z 322
—3. UDP Hexanolamine (internal standard) – the monitored MRM transitions were m/z 502 to m/z 258
—–b(MRM = multiple reaction monitoring in LC/MS/MS)
Notes: Sugar Nucleotides among other metabolites are an important group of compounds to be analyzed when one is trying to understand cellular response to genetic or environmental perturbations.
No 61 Sugar Nucleotides Analyzed with LCMS/MS pdf 0.2 Mb Download File