What inside diameter ID of standard tubing is most suitable for HPLC mobile phase – FAQ

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ARTICLES, APPNOTES, FAQ (9289)
- AppNotes Library (1888)
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- Frequently Asked Questions?? (3402)
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April 27, 2012
Bill Ciccone

ARTICLES, APPNOTES, FAQ / Chromatography Accessories & Supplies Information / Fittings & Tubing for HPLC FAQ / Frequently Asked Questions?? / HPLC Tips and Suggestions / Tubing Information

There really is no standard size and this is truly a preference to meet your method objectives.

For HPLC, on the high pressure side, 1/16th inch outer diameter OD is most commonly used.
The low pressure side of the HPLC instrument mostly uses either the 1/16th inch or 1/8th inch OD depending on where you want to put the tubing on the instrument.

Depending on your column internal diameter (ID), your flow rate you want to use, your pump and detector capability, you can select from the following ID: 0.005″, 0.007″, 0.010″ or 0.020″. The most commonly used ID is 0.007″ for modern HPLC instruments with standard 4.6mm or 3.0mm ID columns.

Click HERE for HPLC tubing ordering information and pictures

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