Performance goals or objectives for a routine HPLC method – Tips & Suggestions

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ARTICLES, APPNOTES, FAQ (9292)
- AppNotes Library (1888)
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April 22, 2012
Bill Ciccone

ARTICLES, APPNOTES, FAQ / Frequently Asked Questions?? / General Information / HPLC Primer / HPLC Suggestions and Information / HPLC Tips and Suggestions / Reversed Phase Columns FAQ

You should define your objectives as loosely as possible for resolution, tailing, precision, LOQ and linearity goals.

Also include such important factors as maximum analysis time, sample prep complexity factors and cost. You may want to also develop your methods to be LCMS compatible.

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