Should I filter my HPLC solvents and buffers before use – FAQ

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ARTICLES, APPNOTES, FAQ (9289)
- AppNotes Library (1888)
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- Frequently Asked Questions?? (3402)
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April 22, 2012
Bill Ciccone

ARTICLES, APPNOTES, FAQ / Cogent Column Filters - FAQ / Filter Degasser FAQ / Filtration FAQ / HPLC Columns FAQ / HPLC Suggestions and Information / HPLC Tips and Suggestions / Mobile Phase FAQ / Syringe Filters and Filtration Information / Syringe Filters FAQ / Trouble Shooting Suggestions

YES! We strongly recommend it.

The most common cause of excessive back pressure is due to the accumulation of particulates and or permanently adsorbed materials at the top of the HPLC column. This could be precipitate or undissolved particles which can come from your samples or your mobile phase or shredded pieces of pump seals.

The best way to minimize accumulation on your column is to filter your samples, solvents and buffers with a .45µm or a 0.2µm syringe filter or through a filter flask before use. This should give you more column lifetime.

Another way to minimize this build up is to use either an in line solvent / mobile phase filter, a guard column and or a Cogent Column Filter. The disposable filters devices are low cost and easy to use without disrupting your chromatographic results. Click HERE for ordering information on the column filters.

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