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Why does the metal content of a silica particle contribute to the peak shape of my chromatogram in HPLC – FAQ

KB Home/ARTICLES, APPNOTES, FAQ /ARTICLES, APPNOTES, FAQ / HPLC Suggestions and Information / HPLC Tips and Suggestions / ARTICLES, APPNOTES, FAQ / Frequently Asked Questions?? / HPLC Columns FAQ / Reversed Phase Columns FAQ / ARTICLES, APPNOTES, FAQ / Frequently Asked Questions?? / HPLC Columns FAQ / TYPE-C in General FAQ / Why does the metal content of a silica particle contribute to the peak shape of my chromatogram in HPLC – FAQ

April 22, 2012
Bill Ciccone

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ARTICLES, APPNOTES, FAQ / HPLC Tips and Suggestions / Reversed Phase Columns FAQ / TYPE-C in General FAQ

The electron withdrawing properties of trace metals in standard HPLC silica particles enhances the negativity of surface silanol groups and contributes to peak tailing when high levels of trace metals are present.

NOTE: This much less of a concern for TYPE-C™ silica.

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