You can use up to 100ml and sometimes more, in a single 25mm AQ™ syringe filter is you are using an aqueous solvent and your sample is not cloudy.
However, the best way to determine if a filter should be replaced from service is when you get a very fast drop in back pressure or a constant rise in back pressure. Both indicate it is time to replace them. Reusing the same syringe filters for multiple samples is never recommended due to carry over problems.
Click HERE for syringe filters ordering information and pictures.
MicroSolv filters equivalency study pdf Download File