You can use up to 100ml in a single 25mm syringe filter is you are using an aqueous solvent and your sample is not cloudy. However, the best way to determine if the filter should be replaced is when you get either a very fast drop in back pressure or a constant rise in back pressure. Both indicate it is time to replace them. Reusing the same syringe filters for multiple samples is never recommended due to carry over problems.
MicroSolvFiltersEquivalency.pdf 26.9 Kb Download File