Do different retention times for peaks in the Resolution Test Mixture on different systems impact the integrity of my data – FAQ

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ARTICLES, APPNOTES, FAQ (9292)
- AppNotes Library (1888)
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April 7, 2012
Bill Ciccone

ARTICLES, APPNOTES, FAQ / Frequently Asked Questions?? / HPLC Qualification / Qualification FAQ / Qualification Information / Qualification Tech Information / Trouble Shooting Suggestions

No impact at all.

There will certainly be mobile phase preparation differences, aging of columns, etc. The HSQ Kit™ instructions call for adjustment of the mobile phase to meet system suitability, which for example in our C of A, calls to adjust the mobile phase to achieve a 4-5 minute retention time window. Keep in mind that the C o A merely confirms that the column was tested, and met specs when it left our facility.

Click HERE for PQ and HSQ Kit ordering information and pictures.

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