Injection volume suggestions for Cogent TYPE-C HPLC columns – Tips & Suggestions

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ARTICLES, APPNOTES, FAQ (9292)
- AppNotes Library (1888)
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April 2, 2012
Bill Ciccone

ARTICLES, APPNOTES, FAQ / HPLC Tips and Suggestions / Trouble Shooting Suggestions / TYPE-C in General FAQ

When using an analytical Cogent TYPE-C™ column it is recommended to inject 1uL to 10uL of the sample which is prepared either in a diluent that matches, as closely as possible, the starting mobile phase composition of a gradient method or the method’s isocratic mobile phase.

Larger volume injections that are performed on the columns are probably overloading the columns.

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