Will “phosphate” precipitate in an HPLC column? – MicroSolv Technology Corporation

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April 1, 2012
jimmy

ARTICLES, APPNOTES, FAQ / HPLC Tips and Suggestions

Phosphoric Acid will not precipitate in the columns.

Sodium phosphate (which is not recommended) but which is often used as a buffer, will precipitate in the columns and should be avoided as a storage solvent for all HPLC Columns.

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1) Why use buffers during analysis and acid buffers such as the phosphoric acid for column storage solvents for Bidenate C18 columns? Will phosphate precipitate in the column?

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