Common Artificial Sweetener

Sucralose does not exhibit significant UV absorption, hence detection of this analyte may be suited to Refractive Index (RI) as an alternative. Both approaches are shown in this application note, where UV and RI detector modules where attached in series. The relatively high concentration used in this example allowed for adequate detection in either case. These conditions were adapted from the USP assay method. A slight modification in the Water content was made to obtain higher retention.

Peak:
Sucralose

Method Conditions
Column: Cogent Bidentate C18™, 4µm, 1 00Å
Catalog No.: 40018-75P
Dimensions: 4.6 x 50 mm
Mobile Phase: 90% DI Water / 10% Acetonitrile (v/v)
Injection vol.: 2µL
Flow rate: 1.0 mL/minute
Detection:
Fig. A: Refractive Index
Fig. B: UV 200 nm
Sample: 5.0 mg/mL Sucralose reference standard in diluent of 85% DI Water / 15% Acetonitrile.
t0: 1.0 minute

Note: The discovery of Sucralose is quite serendipitous. Researcher Shashikant Phadnis was asked by his adviser Leslie Hough to “test” a particular chlorinated sugar compound they had been studying. However, Phadnis misheard him and thought he was being asked to “taste” it instead! Upon doing so, he found it to be much sweeter than ordinary table sugar.

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