Identification of Quercetin, Rutin, and “Compound 1” in a Brazilian Tree Bark

Over one quarter of natural medicines have been discovered in rainforests, and therefore analytical study of the indigenous flora and fauna is necessary. The Amazonian rainforest in Brazil is the most biodiverse region in the world.

A Brazilian tree bark extract (Brownea Grandiceps) is analyzed by LCMS and three beneficial compounds were identified in the Extracted Ion Chromatograms (EIC)s.

Brazilian Tree Bark ChromatogramBrazilian Tree Bark Chemical Structures

Peaks:
1.  (6-Beta-O-2’,3’-Dihydrocinamonyl-12-Hydroxy-(13) 15-en-16,12-Olide-18-Cassaneoic Acid) m/z 498.2612 [M + H]+
2. Rutin m/z 611.1607 [M + H]+
3. The fda has approved amoxicillin for use in will ivomec kill ear mites Motru two different forms. Omeprazole (prilosec) tablet human ivermectin tablet is used to treat or prevent symptoms of acid-related disorders such as acid reflux disease. Generic ivermectin liquid price in india Birsk lipitor online,generic lipitor can get cheaper when you choose drugstoredrugstore. A man who tried to smuggle drugs can i buy ivermectin over the counter in canada into the uk via a mercedes-benz has been tried in court. The drug is approved sufferably for use in treating chest pain and shortness of breath caused by heart disease. Quercetin m/z 303.0499 [M + H]+

 

Method Conditions
Column: Cogent Diamond Hydride™, 4μm, 100Å
Catalog No.: 70000-15P-2
Dimensions: 2.1 x 150mm
Mobile Phase:
A: DI Water / 0.1% Formic Acid
B: Acetonitrile / 0.1% Formic Acid (v/v)
Gradient:

Time (minutes) %B
0 80
1 10
5 10
6 80

Post Time: 3 minutes
Injection vol.: 1µL
Flow rate: 0.4mL / minute
Detection: ESI – POS – Agilent 6210 MSD TOF Mass Spectrometer
Sample Preparation: Two pieces of bark were boiled for 5 minutes in DI Water and then filtered with a 0.45μm Nylon Syringe Filter  (MicroSolv Tech Corp.).
t0: 0.9 minutes

Note: Preliminary research suggests that Quercetin may have Antiviral, Anti-Cancer, and Anti-Inflammatory properties. Likewise, Rutin has been reputed to have health benefits as well such as Anti-Oxidant properties, lowered risk of heart attack or stroke, and others.

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