Herbicide and Metabolites by LCMS – AppNote – MicroSolv Technology Corporation

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ARTICLES, APPNOTES, FAQ (9289)
- AppNotes Library (1888)
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- Frequently Asked Questions?? (3402)
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February 16, 2015
dcatri

AppNotes AgroChem / AppNotes ANP/HILIC Methods / AppNotes Cogent Diamond Hydride / AppNotes Library / AppNotes Listed by Discipline / ARTICLES, APPNOTES, FAQ

Glufosinate, N-Acetylglufosinate, and Glufosinate Propanoic Acid

Analysis of these Compounds can be problematic with other methods and poor peak shape may occur. In contrast, the Peaks obtained in this method are very sharp and symmetrical and can be applied to food products containing these types of Compounds.

Peaks:
1. Glufosinate m/z 180.0431 [M-H]–
2. N-Acetylglufosinate m/z 222.00 [M-H]–
3. Glufosinate Propanoic Acid m/z 151.00 [M-H]–

Method Conditions
Column: Cogent Diamond Hydride™, 2.2μm, 120Å
Catalog No.: 70200-05P-2
Dimensions: 2.1 x 150mm
Mobile Phase:
—A: DI Water / 10mM Ammonium Acetate
—B: 95% Acetonitrile / 5% DI Water / 10mM Ammonium Acetate (v/v)
Gradient:

Time (minutes)	%B
0	90
1	90
1.2	5
5	5
6	90

Post Time: 3 minutes
Flow rate: 0.4 mL/minute
Detection: ESI – NEG – Agilent 6210 MSD TOF Mass Spectrometer
Injection vol.: 1μL
Sample Preparation: Glufosinate (1720.64 ppm), N-Acetylglufosinate (639.2 ppm), and Glufosinate Propanoic Acid (1302.5 ppm) stock solutions were diluted 1:100 with 4:1 DI Water: Methanol
t0: 0.6 minutes

Note: Glufosinate is an Herbicide which acts by interference with the Ammonia detoxification metabolic pathway. Trade names of formulations featuring the compound include Rely®, Finale®, and Ignite®.

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Herbicide and Metabolites by LC-MS pdf Download File

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