LSD and Codeine – AppNote – MicroSolv Technology Corporation

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ARTICLES, APPNOTES, FAQ (9292)
- AppNotes Library (1888)
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February 4, 2015
dcatri

AppNotes ANP/HILIC Methods / AppNotes Cogent Diamond Hydride / AppNotes Library / AppNotes Metabolites / AppNotes Pharmaceuticals / ARTICLES, APPNOTES, FAQ

LCMS Method for Spiked Urine Sample

LSD and codeine may be present in Urine samples and methods are needed for their detection in these matrices. The extracted Ion Chromatograms (EICs) for both analytes are shown in the figure and illustrate excellent chromatographic as well as Mass Spectral Separation. The Method in this AppNote produces high Efficiency for both Peaks.

Peaks:
1. LSD 324.207 m/z [M + H]+
2. Codeine 300.1594 m/z [M + H]+

Method Conditions
Column: Cogent Diamond Hydride 2.o™, 2.2μm, 120Å
Catalog No.: 70200-05P-2
Dimensions: 2.1 x 50mm
Mobile Phase:
—A: DI Water / 0.1% Formic Acid (v/v)
—B: Acetone / 0.1% Formic Acid (v/v)
Gradient:

Time (Minutes)	%B
0	85
3	30
5	30
6	85

Flow rate: 0.4 mL/minute
Detection: ESI – POS – Agilent 6210 MSD TOF Mass Spectrometer
Injection vol.: 1μL
Sample Preparation: Urine spiked with 5ppm LSD and Codeine standard solutions

Note: Lysergic Acid Diethylamide (LSD) was synthesized by Swiss chemist Albert Hofmann while researching Ergot Alkaloid Derivatives. It is highly potent and produces significant psychedelic effects when ingested. It is currently a Schedule I drug in the U.S. Codeine is an opiate used for analgesic, antitussive, and other effects. It may be obtained from over-the-counter formulations like Cough Syrup and therefore has potential for abuse.

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