Vanillic Acid and 6′-O-Feruloylsucrose Analyzed with LCMS – AppNote

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ARTICLES, APPNOTES, FAQ (9292)
- AppNotes Library (1888)
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January 7, 2014
Bill Ciccone

AppNotes Cogent Phenyl Hydride / AppNotes Food and Beverages / AppNotes Library / AppNotes Reversed Phase Methods / ARTICLES, APPNOTES, FAQ

Rice Extract Sample Containing Vanillic Acid and Feruloylsucrose
Click HERE for Column Ordering Information.

When commercial Rice Extracts were analyzed, only Vanillic Acid (Peak 1) was found in one out of 3 Extracts and 6′-O-Feruloylsucrose (peak 2) was found in all 3 extracts. The results were confirmed by analyzing spiked Rice Extract samples. The analysis was done in a single run by switching polarity between positive and negative ionization mode.

According to the literature [1], a Rice Extract should contain following compounds: Gallic Acid, Vanillic Acid, p-Coumaric Acid, Ferulic Acid, 3,5 Dimethoxy-4-Hydroxycinnamic Acid, Syringic Acid, Caffeic Acid, and 6′-O-Feruloylsucrose.

Peaks:
1. Vanillic Acid 167 m/z [M-H]–
2. 6′-O-Feruloylsucrose 178 m/z [M-C12H22O11+H]+

Method Conditions
Column: Cogent Phenyl Hydride™, 4μm, 100Å
Catalog No.: 69020-05P-2
Dimensions: 2.1 x 50mm
Mobile Phase:
—A: DI Water with 0.1% Formic Acid (v/v)
—B: Acetonitrile with 0.1% Formic Acid (v/v)
Gradient:

Time (minutes)	%B
0	10
5	20
6	20
7	10

Post Time: 3 minutes
Injection vol.: 1μL
Flow rate: 0.3mL / minute
Detection: ESI – NEG, POS – PerkinElmer Flexar SQ 300 Mass Spectrometer
Sample Preparation: A commercially available Rice Extract was analyzed.
t0: 0.6 minutes

Note: Rice is a staple food in many countries. It contains phenolic compounds which have anticancer, antioxidant, and antimutagenic effects. It is important to analyze rice extracts to confirm the content of the phenolic compounds in rice.

[1] S. Tian, K. Nakamura, T. Cui, H. Kayahara, J. Chromatogr. A, 1063 (2005) 121-128.

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