Lansoprazole Capsule Analyzed with HPLC – AppNote – MicroSolv Technology Corporation

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ARTICLES, APPNOTES, FAQ (9292)
- AppNotes Library (1888)
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November 2, 2013
Bill Ciccone

AppNotes Cogent Bidentate C18 / AppNotes Library / AppNotes Pharmaceuticals / AppNotes Reversed Phase Methods / ARTICLES, APPNOTES, FAQ

Separation from matrix peaks

Lansoprazole is separated from several Matrix Components in this simple assay method. The Mobile Phase is LC-MS compatible so the method could be applied to more complex samples such as plasma. Five runs are shown in the figure to illustrate the repeatability of the data.

Peak:
Lansoprazole

Method Conditions
Column: Cogent Bidentate C18™, 2.2µm, 120Å
Catalog No.: 40218-05P-2
Dimensions: 2.1 x 50 mm
Mobile Phase:
– A: DI Water / 0.1% Formic Acid (v/v)
– B: Acetonitrile / 0.1% Formic Acid (v/v)
Gradient:

Time (minutes)	%B
0	10
1	10
6	70
7	10

Injection vol.: 0.2 μL
Flow rate: 0.4mL / minute
Detection: UV @ 285 nm
Sample Preparation: 15mg strength Lansoprazole capsule contents were ground and added to a 25mL volumetric flask. A portion of 50/50 Solvent A / Solvent B was added and the flask was sonicated 10 minutes. Then it was diluted to mark and mixed. A portion was filtered through a 0.45µm Nylon Syringe Filter (MicroSolv Tech Corp.).
t0: 0.5 minutes

Note: Lansoprazole is a proton-pump inhibitor used for acid reducing effects. It is available over-the-counter by Novartis under the trade name Prevacid, as well as generic versions.

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