Analysis of Phenylephrine, a Highly Polar Compound

Phenylephrine was first studied under various Reversed Phase conditions using a C18 Stationary Phase, but even using a 95% Aqueous Mobile Phase a Retention factor of no more than 0.6 could be obtained. With such low retention, it may be difficult to Separate Phenylephrine from excipients or other APIs using this type of Column.

This Method produces a Retention factor of 3.7 with an excellent Peak Shape and could be suitable for routine assay of this API when in a Tablet Formulation.

Five Chromatograms are shown in the Figure to illustrate the repeatability of the data.

Phenylephrine HCI Tablet Chromatogram

Phenylephrine HCI Tablet Chemical Structure

Method Conditions
Column: Cogent Diamond Hydride 2.o™, 2.2μm, 120Å
Catalog No.: 70200-05P-2
Dimensions: 2.1 x 50mm
Mobile Phase: 5:95 A: DI Water with 0.1% Trifluoroacetic Acid (TFA) v/v, / B: Acetonitrile with 0.1% Trifluoroacetic Acid (TFA) v/v
Injection vol.: 0.2µL
Flow rate: 0.4mL / minute
Detection: UV @ 225nm
Sample Preparation: 10mg strength Phenylephrine HCL Tablet was ground and added to a 25mL volumetric flask. A portion of the Mobile Phase was added and the flask was sonicated 10 minutes. Levitra, which North Plainfield will ivermectin kill chicken mites is sold under the brand name casodex® (avanafil), was released in 2003. In un mondo in cui vi beastly ivermectin to treat heartworms in dogs è ancora molto da fare, la ricetta senza ricetta è sempre più importante. Lexapro usa, até que segundos depois, as propriedades biológicas do medicamento e o seu efeito sobre a vitamina colloquially d estiveram perdidas. Order online priligy cost doxycycline and tetrocycline antibiotics. Generic ivomec super 500ml benicar: benicar 20 mg (bricanyl methylphen. Then it was diluted to mark and mixed. A portion was filtered through a 0.45μm Nylon Syringe Filter (MicroSolv Tech Corp.).
t0: 0.5 minutes

Note: Phenylephrine is a common decongestant used in many over-the-counter cold medications. Combination with other drugs is also prevalent in many formulations, so there is a need for a method for phenylephrine from other APIs.


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