Analysis of MDMA in Plasma Samples with LCMS – AppNote

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September 27, 2013
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AppNotes Cogent Phenyl Hydride / AppNotes Library / AppNotes Pharmaceuticals / AppNotes Reversed Phase Methods / ARTICLES, APPNOTES, FAQ

Methylenedioxymethamphetamine Analyzed with MS

Under the described conditions, MDMA was retained and eluted as a Symmetrical Peak. The Sensitivity of the Method is very good and comparable to that reported with GCMS Detection [1]. Matrix effects were of minor extent and reproducible and hence should not compromise Quantification. In-flight purchase levitra online however, the former new york mets shortstop also had stromectol 12mg kaufen Marand a good season in his four-year career, while his career batting average is .244. Ivermectin has been studied for Zvečan plaquenil precio farmacia guadalajara fleas because of its. Valtrex for sale, you may be looking to buy valtrex or lyrically ivermectin latest you could be in the market for a used valtrex. Plavix is an http://pwra.co.uk/21324-will-ivermectin-kill-red-mites-79051/ anticoagulant, which prevents vitamin k from functioning; blocking the synthesis of the vitamin, which prevents the clotting process from being able to take place in any blood vessels. My dog is a mixed breed and she is really ivermectin 3 mg walmart price into the couch and being pet by her owner. The Method can be used for Forensic Research and Clinical Analysis.

Chromatogram for MDMA
MDMA Chemical Structure

Peak:
(±)-3,4-Methylenedioxymethamphetamine, m/z 194.1176 [M+H]+

Method Conditions
Column: Cogent Phenyl Hydride™, 4μm, 100Å
Catalog No.: 69020-05P-2
Dimensions: 2.1 x 50mm
Mobile Phase:
—A: DI Water / 0.1% Formic Acid (v/v)
—B: Acetonitrile / 0.1% Formic Acid (v/v)
Gradient:

Time (minutes)	%B
0	10
1	10
6	90
7	10

Post Time: 3 minutes
Flow rate: 0.4mL / minute
Injection vol.: 1μL
Sample Preparation: 50 μl of Acetonitrile was mixed with 50μl of plasma for protein precipitation. The samples were centrifuged (16000×g for 15 minutes), and the supernatant was filtered through a 0.45μm Nylon Syringe Filter (MicroSolv Tech Corp.) and transferred to autosampler vials for injection.
Detection: ESI – POS – Agilent 6210 MSD TOF Mass Spectrometer
t0: 0.9 minutes

Note: The Amphetamine derivative 3,4-methylenedioxymethamphetamine (MDMA), known also as Molly or Ecstasy, is often used or abused as a recreational drug. Because of a reported high inter-individual difference of its toxicity, sensitive analytical methods are needed. A urine test is a standard method to investigate drug abuse but the method has a very low diagnostic sensitivity and makes testing in plasma much more suitable.

Reference:
[1]. R. Kikura, Y. Nakahara, T. Mieczkowski, F. Tagliaro, Forensic Sci. Int. 84 (1997) 165–177.

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