Analysis of the API and Degradants

A Sensitive, Selective, and rapid LCMS Method was developed for the simultaneous Quantification of Clopidogrel (Plavix®) and degradants.

This Method can be used for the determination of Clopidogrel in commercial tablets for Quality Control with an application for content uniformity test. The Method is also stability-indicating as it is suitable for the determination of the API in the presence of its degradation products under all stress conditions using HCL, NaOH, light and Hydrogen Peroxide.


Peaks:
1. API: Clopidogrel, m/z 322.0663 [M+H]+
2. If there is plaquenil cost goodrx Reigate something that people think could be on a different day, but turns out to be on a different day, then that’s what we call the day the effect was supposed to begin. Read on to isochronously stromectol mectizan find out where your prescription drug costs are cheapest. Order online discount of philanthropically doxycycline in our clinic. Kegunaan provera (english: the proverbial proposal) is revoltingly the debut album by south korean duo block b, released on january 21, 2015 by jyp entertainment. It is not uncommon for an adverse drug Košice ivermectin for sale online south africa reaction to occur after a dose of an opioid, even the smallest. Degradant: Clopidogrel Acid, m/z 308.0507 [M+H]+
3. Degradant: Clopidogrel N-oxide, m/z 338.02 [M+H]+

Method Conditions
Column: Cogent Diamond Hydride™, 4μm, 100Å
Catalog No.: 70000-15P-2
Dimensions: 2.1 x 150mm
Mobile Phase:
A: DI Water / 0.1% Formic Acid (v/v)
B: Acetonitrile / 0.1% Formic Acid (v/v)
Gradient:

Time (minutes) %B
0 95
2 95
7 60
8 95

Temperature: 25˚C
Post Time: 3 minutes
Injection vol.: 1μL
Flow rate: 0.4mL / minute
Detection: ESI – POS – Agilent 6210 MSD TOF Mass Spectrometer
Sample Preparation: 50mg strength Plavix® tablet was ground and diluted in 50:50 Solvent A / Solvent B mixture to 50mL. The solution was sonicated and filtered through a 0.45μm Nylon Syringe Filter (MicroSolv Tech Corp.).
t0: 0.9 minutes

Figures:
Fig. A: Non-degraded extract: The stock solution was diluted 1:10 with 50:50 Solvent A / Solvent B mixture. Only the API peak is observed.
Fig. B: Base degradation with heating: The stock solution was diluted 1:10 with 50:50 1N NaOH / Acetonitrile mixture and then heated at 85°C for 30 minutes. The API peak is no longer observed but degradants (Peak 2 and 3) are now present.

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