Retention of a Polar, Sulfonate Compound

As a highly polar compound, Taurine is often difficult to retain by Reversed Phase Chromatography. The Peak Shape and Retention obtained with this Aqueous Normal Phase (ANP) LCMS Method is greatly improved and is suitable as a starting point for further analyses of Taurine, in a variety of Samples such as beverages or even biological matrices.


Peak:
Taurine, m/z 126.0219 [M+H]+

Method Conditions
Column: Cogent Diamond Hydride™, 4μm, 100Å
Catalog No.: 70000-15P-2
Dimensions: 2.1 x 150mm
Mobile Phase:
A: DI Water with 0.1% Formic Acid (v/v)
B: Acetonitrile with 0.1% Formic Acid (v/v)
Gradient:

Time (minutes) %B
0 95
1 95
6 30
7 30
8 95

Post Time: 3 minutes (3.3 column volumes)
Injection vol.: 2μL
Flow rate: 0.4mL / minute
Detection: ESI – POS – Agilent 6210 MSD TOF Mass Spectrometer
Sample Preparation: 10mg / L of Taurine reference standard was added to a diluent of 50:50 Solvent A / Solvent B.
t0: 0.9 minutes

Note: Taurine is added to many popular energy drinks. It is found naturally in animal tissues and is a major constituent of bile.

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