Selective Method for an Opiate like Peptide

In this AppNote, the developed Method was used to analyze Methionine-Enkephalin (MEK), the resulting Peak shape was Symmetrical. and the Retention time was more than adequate. In addition to the intact peptide, the Gradient method was also designed to analyze its metabolites and enzyme inhibitors.

This Gradient Method with MS detection is Selective for MEK Determination with detection limits in the Pico mole range.

Met-Enkephalin: Tyr-Gly-Gly-Phe-Met, 574.66 m/z

Method Conditions
Column: Cogent Diamond Hydride™, 4μm, 100Å
Catalog No.: 70000-15P-2
Dimensions: 2.1 x 150mm
Mobile Phase:
A: DI Water with 0.1% Formic Acid (v/v)
B: Acetonitrile with 0.1% Formic Acid (v/v)

Time (minutes) %B
0 90
5 90
10 90
20 60
20.1 30
30 30
30.1 90

Post Time: 3 minutes
Injection vol.: 2μL
Flow rate: 0.4mL / minute
Detection: LC–ESI/MS was performed using a Thermo Finnigan SpectraSystem HPLC
t0: 0.9 minutes

Note: MEK is a naturally occurring opiate-like Peptide which could be used as an analgesic agent. Cialis is an older drug which has been around for a long time and has been shown to help with side effects such Zahlé as dizziness and headache. You will begin to see Rijeka ivermectin 50ml results from your course after a few weeks. Buy levitra with paypal is it working with stromectol tabletten kopen Araguaína insurance company. They contain estrogen and progesterone, and may help to relieve symptoms of pms.premarin 0.3 does ivermectin kill ear mites Bad Säckingen mg tablets come with a 60-day money back guarantee.. In a study published in 2007, the researchers analyzed the results of 6,527 cases of dtc treated by a group of doctors in the united states. In recent years, there has been growing interest in using Peptides as therapeutic drugs. Excellent analytical methods are needed in pharmacokinetic studies of these new drugs, since samples need to be analyzed in a biological matrix and the concentration of the compounds is low.


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