Streptomycin Analyzed with HPLC – AppNote – MicroSolv Technology Corporation

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ARTICLES, APPNOTES, FAQ (9292)
- AppNotes Library (1888)
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November 19, 2012
Bill Ciccone

AppNotes ANP/HILIC Methods / AppNotes Cogent Diamond Hydride / AppNotes Library / AppNotes Metabolites / AppNotes Pharmaceuticals / ARTICLES, APPNOTES, FAQ

Retention of a Highly Polar Antibiotic

Streptomycin is very polar and difficult to retain with Reversed Phase methods using conventional Columns. In this Method, retention is achieved with Separation of the Streptomycin from two impurities found in the Standard Solution. Furthermore, the numerous amine groups do not cause tailing of Peak shape as with standard silica-based Columns.

Data from two Column lots shown in the figure illustrates the reproducibility of the Method and it robustness.

Peaks:
1. Impurity
2. Streptomycin
3. Impurity

Method Conditions
Column: Cogent Diamond Hydride™, 4µm, 100A
Catalog No.: 70000-7.5P
Dimensions: 4.6 x 75mm
Mobile Phase:
—A: DI Water / 0.1% Trifluoroacetic Acid (v/v)
—B: Acetonitrile/ 0.1% Trifluoroacetic Acid (v/v)
Gradient:

Time (minutes)	%B
0	95
1	95
6	40
7	95

Post Time: 3 minutes
Injection Vol.: 1μL
Flow Rate: 1.0mL / minute
Detection: UV @ 205nm
Sample Preparation: 1.0mg / mL Streptomycin Sulfate reference standard in Solvent A diluent.
t0: 0.9 minutes

Note: Streptomycin is an aminoglycoside antibiotic derived from the Actinobacterium Streptomyces Griseus. Streptomycin was isolated in 1943 and was the first antibiotic to treat tuberculosis.

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