API Separation from Matrix Component
This Method for Analysis of Famotidine Tablets is easy to perform and produces a Symmetrical Peak Shape for the API. This compound has numerous amines which can be problematic in terms of Peak Shape with conventional Columns. Separation from a component from the tablet extract matrix is obtained as well, illustrating specificity of the Method.
Reproducibility is shown by the overlay of runs from two different Column lots.
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Column: Cogent Diamond Hydride™, 4μm, 100Å
Catalog No.: 70000-7.5P
Dimensions: 4.6 x 75mm
—A: DI Water with 0.1% Trifluoroacetic Acid (TFA) v/v
—B: Acetonitrile with 0.1% Trifluoroacetic Acid (TFA) v/v
Post Time: 3 minutes
Injection vol.: 1μL
Flow rate: 1.0mL / minute
Detection: UV @ 265nm
Sample Preparation: 10mg strength Famotidine tablet was ground and added to a 25mL volumetric flask. A portion of 50:50 Solvent A / Solvent B diluent was added and the flask was sonicated 10 minutes. It was then diluted to mark and filtered with a 0.45μm Nylon Syringe Filter (MicroSolv Tech Corp.).
t0: 0.9 minutes
No 221 Famotidine Tablet Analyzed with HPLC pdf 0.4 Mb Download File