Famotidine Tablet Analyzed with HPLC – AppNote – MicroSolv Technology Corporation

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ARTICLES, APPNOTES, FAQ (9292)
- AppNotes Library (1888)
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August 12, 2012
Bill Ciccone

AppNotes ANP/HILIC Methods / AppNotes Cogent Diamond Hydride / AppNotes Library / AppNotes Pharmaceuticals / ARTICLES, APPNOTES, FAQ

API Separation from Matrix Component

This Method for Analysis of Famotidine Tablets is easy to perform and produces a Symmetrical Peak Shape for the API. This compound has numerous amines which can be problematic in terms of Peak Shape with conventional Columns. Separation from a component from the tablet extract matrix is obtained as well, illustrating specificity of the Method.

Reproducibility is shown by the overlay of runs from two different Column lots.

Famotidine Tablet Chromatogram
Famotidine Chemical Structure
Peaks:
1. Matrix Component
2. Famotidine

Method Conditions
Column: Cogent Diamond Hydride™, 4μm, 100Å
Catalog No.: 70000-7.5P
Dimensions: 4.6 x 75mm
Mobile Phase:
—A: DI Water with 0.1% Trifluoroacetic Acid (TFA) v/v
—B: Acetonitrile with 0.1% Trifluoroacetic Acid (TFA) v/v
Gradient:

Time (minutes)	%B
0	95
2	95
6	50
7	95

Post Time: 3 minutes
Injection vol.: 1μL
Flow rate: 1.0mL / minute
Detection: UV @ 265nm
Sample Preparation: 10mg strength Famotidine tablet was ground and added to a 25mL volumetric flask. A portion of 50:50 Solvent A / Solvent B diluent was added and the flask was sonicated 10 minutes. It was then diluted to mark and filtered with a 0.45μm Nylon Syringe Filter (MicroSolv Tech Corp.).
t0: 0.9 minutes

Attachment

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