Ranitidine Tablet Analyzed with HPLC – AppNote – MicroSolv Technology Corporation

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ARTICLES, APPNOTES, FAQ (9289)
- AppNotes Library (1888)
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- Frequently Asked Questions?? (3402)
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November 8, 2012
dcatri

AppNotes ANP/HILIC Methods / AppNotes Cogent Diamond Hydride / AppNotes Library / AppNotes Pharmaceuticals / ARTICLES, APPNOTES, FAQ

Excellent Peak Shape of API From an OTC Tablet

Ranitidine has several amine functional groups that can contribute to significant tailing with Reversed Phase Methods. Data shown below uses an extract from a tablet formulation, illustrating how good Peak shape can be obtained. In addition, three Column lots were used to demonstrate the robustness and precision of this Method.

Peaks:
1. Ranitidine
2. Matrix Component

Method Conditions
Column: Cogent Diamond Hydride™, 4μm, 100Å
Catalog No.: 70000-7.5P
Dimensions: 4.6 x 75mm
Mobile Phase:
—A: DI Water / 0.1% Trifluoroacetic acid (v/v)
—B: Acetonitrile / 0.1% Trifluoroacetic acid (v/v)
Gradient:

Time (minutes)	%B
0	95
1	95
6	50
7	95

Post Time: 3 minutes
Injection vol.: 2μL
Flow rate: 1.0mL / minute
Detection: UV @ 313nm
Sample Preparation: 150mg strength Ranitidine HCl tablet was ground and weighed in a 50mL volumetric flask. A portion of 50:50 Solvent A / Solvent B diluent was added and the flask was sonicated 10 minutes. It was then diluted to mark and filtered with a 0.45µm Nylon Syringe Filter (MicroSolv Tech Corp.). The filtrate was diluted 1:100 for injections.
t0: 0.9 minutes

Note: Ranitidine is an acid reducer that is available over-the-counter. It works as a histamine H2-receptor antagonist, in contrast to other acid reducers such as omeprazole which are proton pump inhibitors. It is sold under the trade name Zantac®.

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