Potential Clinical Screening Method for Galactosemia
This Method is useful as a quantitative screening or routine clinical test to detect infants suspected of having a defect of Galactose Metabolism. It can also be used to monitor blood levels of Galactose-1-Phosphate in children with Galactosemia who are on a lactose-free diet.
Galactose-1-phosphate, 259.0224 m/z (M-H)–
Column: Cogent Diamond Hydride™, 4μm, 100Å
Catalog No.: 70000-15P-2
Dimensions: 2.1 x 150mm
—A: DI Water with 0.1% Formic Acid (v/v)
—B: 90:10 Acetonitrile / DI Water with 16.5mM Ammonium Acetate (v/v)
Post Time: 5 minutes
Injection vol.: 1μL
Flow rate: 0.4mL / minute
Detection: ESI – NEG – Agilent 6210 MSD TOF Mass Spectrometer
—Stock Standard: 1mg / mL Galactose-1-phosphate in DI Water, stored at -20°C.
—Working Solution: Stock was diluted 1:100 with 50:50 Acetonitrile / DI Water Solution.
t0: 0.9 minutes
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