Potential Clinical Screening Method for Galactosemia

This Method is useful as a quantitative screening or routine clinical test to detect infants suspected of having a defect of Galactose Metabolism. It can also be used to monitor blood levels of Galactose-1-Phosphate in children with Galactosemia who are on a lactose-free diet.


Peak:
Galactose-1-phosphate, 259.0224 m/z (M-H)–

Method Conditions
Column: Cogent Diamond Hydride™, 4μm, 100Å
Catalog No.: 70000-15P-2
Dimensions: 2.1 x 150mm
Mobile Phase:
A: DI Water with 0.1% Formic Acid (v/v)
B: 90:10 Acetonitrile / DI Water with 16.5mM Ammonium Acetate (v/v)
Gradient:

Time (minutes) %B
0 95
1 95
3 85
6 85
7 75
10 50
12 50
13 30
15 30
15.00 95

Post Time: 5 minutes
Injection vol.: 1μL
Flow rate: 0.4mL / minute
Detection: ESI – NEG – Agilent 6210 MSD TOF Mass Spectrometer
Sample Preparation:
Stock Standard: 1mg / mL Galactose-1-phosphate in DI Water, stored at -20°C.
Working Solution: Stock was diluted 1:100 with 50:50 Acetonitrile / DI Water Solution.
t0: 0.9 minutes

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