Phenolic compounds analyzed and separated with normal phase HPLC – AppNote

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ARTICLES, APPNOTES, FAQ (9289)
- AppNotes Library (1888)
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- Frequently Asked Questions?? (3402)
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May 23, 2012
Bill Ciccone

AppNotes Cogent Silica-C / AppNotes Library / AppNotes Normal Phase / AppNotes Pharmaceuticals / ARTICLES, APPNOTES, FAQ

Cogent Silica-C™ compared to standard silica in normal phase HPLC

The Cogent Silica-C™ column was compared with a different brand and type of silica column for separation of three proprietary, large, substituted phenol compounds using normal phase HPLC .

The Cogent Silica-C™ column provided satisfactory selectivity under isocratic elution conditions. The column displayed typical normal phase elution for the phenols, i.e. retention increased as the amount of the least polar component in the mobile phase increased. In addition, the separation, is extremely reproducible (%RSD 0.2) even though the solvents were not dried in any special way. This makes normal phase HPLC much easier to perform.

Phenolic Chemical Structures

Peaks:
1. Phenolic with Aldehyde
2. Parent Phenolic Compound
3. Phenolic with a Ketone

Method Conditions
Column: Cogent Silica-C™, 4μm, 100Å
Catalog No.: 40000-75P
Dimensions: 4.6 x 75mm
Mobile Phase: 95:5 Hexane / Ethyl Acetate
Injection vol.: 1μL
Flow rate: 1mL / minute
Detection: UV with a Diode Array
Sample: 1mg / mL of proprietary compounds dissolved in the Mobile Phase: Phenolic Compound with an Aldehyde group, Parent Phenolic Compound, Phenolic with a Ketone group were dissolved in the Mobile Phase

Notes: With the Cogent Silica-C™ column, almost all of the Silanols on the Silica surface are substituted with Si-H and water is not strongly associated by the stationary phase. Drying of the Mobile Phase Solvents is not essential and subsequent analyses are very reproducible, day to day as well as run to run. The identity of the Peaks was confirmed using Atmospheric Pressure Chemical Ionization in the positive and negative mode: APCI+, APCIAPP.

Attachment

Phenolic compounds analyzed with normal phase HPLC pdf Download File

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