Analysis of Amino-Caproic Acid by a Simple LCMS Method

6-Amino-N-Caproic Acid is an active pharmaceutical ingredient used in massive thrombolysis and proteolysis secondary to metastatic carcinoma of the prostate. It is also a potent in vitro inhibitor of fibrinolysis. After oral administration 6-Amino-N-Caproic Acid enhances the uptake of labeled Fibrinogen in both the Walker and Murphy tumors.

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Peaks:
1. Uracil m/z 112
2. 6-Amino-N-Caproic Acid m/z 132

Method Conditions
Column: Cogent Bidentate C18™, 4µm, 100Å
Catalog No.: 40018-75P
Dimensions: 4.6 x 75mm
Mobile Phase:
A: 30% DI Water / 70% Acetonitrile / 0.1% Formic Acid
B: 20% DI Water / 80% Acetonitrile / 0.1% Formic Acid
Injection vol.: 2µL
Flow rate: 0.5mL / minute
Detection: Mass Spectrometer – Atmospheric Pressure Chemical Ionization in positive mode: APCI+ Single Ion Monitoring

Note: Mobile phase with 0.1% Formic Acid can be used in the UV detection of 6-Amino-N-Caproic Acid at 206nm.

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