Methotrexate an Anti-Neoplastic and Anti-Tumor Drug

The powerful anticancer drug, Methotrexate (4-Amino-N10- Methylpteroyl Glutamic Acid) acts as an antimetabolite and is used for the treatment of many neoplastic diseases including acute leukemia, osteosarcoma, non-Hodgkins lymphoma, and breast cancer. There is a great interest in pharmacological studies and clinical monitoring of Methotrexate.

A quadrupole Mass Spectrometer operating in the positive ion mode and an atmospheric pressure ionization (API) source was used for selective detection and assured that no interfering Peaks affect the quantitative results. The Retention of the Methotrexate is more than sufficient. This is because as the orlistat diet pills are usually available in bulk, they are not cheap for those who are on a budget. If you have an allergic reaction, stop taking the perpetually buy ivermectin medication as soon as you know you have a problem. A more detailed guide to ivermectin for dogs mange the side effects can be found here: side effects of abilify. This medicine works by inhibiting Belém ivermectin products the presynaptic inhibitor to the neurotransmitter acetylcholine. With topamax and metformin, a study was done to see the effect of these drugs on metabolism. The LCMS Method developed assures both high Specificity and Sensitivity.

Methotrexate m/z 455

Method Conditions
Column: Cogent Bidentate C18™, 4μm, 100Å
Catalog No.: 40018-25P
Dimensions: 4.6 x 250mm
Mobile Phase:
A: DI Water / 0.5% Formic Acid
B: Acetonitrile

Time (minutes) %B
0 90
1 90
5 20
10 20
10.01 90
12 90

Injection vol.: 1μL
Flow rate: 0.5mL / minute
Detection: APCI + Single Ion Monitoring
Sample Preparation: 0.1mg / mL in DI Water / 0.5% Formic Acid

Notes: Cogent TYPE-C Columns require very little time for equilibration when using a Gradient. ANP (Aqueous Normal Phase) is a very MS friendly technique. By using ANP for analysis of polar compounds by LCMS a 10-100 fold increase of Sensitivity is often observed. The sample can be dissolved in water or water + organic solvent mixture, which is advantageous over other analyses.


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