Simple and Fast Analyses of an Important Biogenic Amine
Using this Method to analyze Tryptamine produces excellent Peak Shape and Reproducible Retention Times as shown. It is very polar and very difficult to Retain when using standard C18 Columns.
The baseline in this Chromatogram appears to be noisy but it is due the low UV Wavelength used along with the presence of Formic Acid and Trifluoroacetic Acid (TFA) in the Mobile Phase. The Method was originally developed for LCMS where the additives do not disturb the baseline but enhance ionization of the sample.
Column: Cogent Diamond Hydride™, 4μm, 100Å
Catalog No.: 70000-7.5P
Dimensions: 4.6 x 75mm
—Isocratic Run: 85:15 Solvent B / Solvent A
—A: DI Water / 0.1% Formic Acid/ 0.001% Trifluoroacetic Acid (TFA)
—B: Acetonitrile / 0.1% Formic acid/ 0.001% Trifluoroacetic Acid (TFA)
Flow rate: 1.0mL / minute
Detection: UV @ 223nm
Sample Preparation: Tryptamine Hydrochloride Standard 0.1mg / mL
t0: 0.892 minutes
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No 51 Tryptamine Analyzed with HPLC pdf 0.1 Mb Download File