Maleic & Fumaric Acids Analyzed with LCMS – AppNote

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ARTICLES, APPNOTES, FAQ (9289)
- AppNotes Library (1888)
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- Frequently Asked Questions?? (3402)
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May 15, 2012
Bill Ciccone

AppNotes ANP/HILIC Methods / AppNotes Cogent Diamond Hydride / AppNotes Library / AppNotes Metabolites / ARTICLES, APPNOTES, FAQ

Isobaric Organic Acids Separated and Identified

Low molecular mass, Isobaric Acids were Retained, Separated and Identified in this simple Method. The Separation of the two compounds with identical mass is necessary in order to determine which of the acids is present in the sample matrix by the Mass Spectrometer.

The Method presented was found to be superior to other classical Assays and HPLC Methods, and to be a good choice for the analysis of these compounds.

Peaks:
1. Maleic Acid 115.0031 m/z (M-H)-
2. Fumaric Acid 115.0031 m/z (M-H)-

Method Conditions
Column: Cogent Diamond Hydride™, 4μm, 100Å
Catalog No.: 70000-15P-2
Dimensions: 2.1 x 150mm
Mobile Phase:
—A: DI Water / 0.1% Ammonium Formate
—B: 90% Acetonitrile / 10% DI Water / 0.1% Ammonium Formate
Gradient:

Time (minutes)	%B
0	90
3	90
6	70
7	70
7.1	30
8	30
8.1	90
10	90

Flow rate: 0.4mL / minute
Detection: ESI – neg – Agilent 6210 MSD TOF Mass spectrometer
Sample Preparation: Sample mixture was prepared in 50:50 DI Water / Acetonitrile

Notes: Fumaric and Maleic Acids are impurities found in Tartaric and Malic Acids and their amounts are officially limited by the FDA.

Attachment

No 59 Maleic & Fumaric Acids Analyzed with LCMS pdf 0.2 Mb Download File

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