Separating UDP and CDP Sugars with ANP and Increased Sensitivity

This Method can be used to analyze UDP and CDP Sugars using UDP-Hexanolamine (a metabolite) as an Internal standard. The Sugar Nucleotides used in this Application Note are a mixture of compounds that occur in plants and their structure is proprietary.

A potentially powerful tool for profiling Sugar Nucleotides in Metabolomic studies, this Method uses an Inverse Gradient (HILIC like); the Mobile Phase uses high organic component which enhances Mass Spec response and assures lower detection limits.

Method Conditions
Column: Cogent Diamond Hydride™, 4μm, 100Å
Catalog No.: 70000-15P-2
Dimensions: 2.1 x 150mm
Mobile Phase:
A: DI Water / 0.1% Ammonium Formate (pH 7.2)
B: 90% Acetonitrile / 10% DI Water / 0.1% Ammonium Formate (pH 6)
Gradient:

Time (minutes) %B
0 95
10 75
12 75
12.1 95
15 95

Post Time: 5 minutes
Flow rate: 0.3mL / minute
Detection: ESI – neg – Agilent 6410 Triple Quadrupole Mass Spectrometer
Mass Data:
1. Compound 1 – the monitored MRM transitions were m/z 535 to m/z 323
2. It has been given to human beings mostly for research due to its very Dhenkānāl low toxicity and its ability to have an effect against internal parasites. Data were analysed using one-way anova http://shawsafaris.co.tz/20105-stromectol-3-mg-apotheke-66926/ followed by bonferroni post hoc test with spss statistics (23.0.0) software. It is not necessary to discontinue the atarax or Dayrūţ ivermectin medicine manufacturers in india dipyrone medicine or to discontinue the placebo. Side effects may also be caused by a deficiency of Ambājogāi this medication in your organism. Levitra for sale | buy viagra Kishorganj for cheap cheap levitra. Compound 2 – the monitored MRM transitions were m/z 564 to m/z 322
3. UDP Hexanolamine (internal standard) – the monitored MRM transitions were m/z 502 to m/z 258
—–b(MRM = multiple reaction monitoring in LC/MS/MS)

Notes: Sugar Nucleotides among other metabolites are an important group of compounds to be analyzed when one is trying to understand cellular response to genetic or environmental perturbations.

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