Robust Separation of API from USP Internal Standard

The USP Assay Method for Alprazolam uses a bare Silica Column and a complex Mobile Phase consisting of Acetonitrile, Chloroform, Butyl Alcohol, and Acetic Acid. In this Method, a simple LCMS compatible Mobile Phase is used and produces excellent Peak shapes for both the API and its USP internal standard.

Furthermore, a resolution of 4.3 was obtained between the two Peaks, which meets the USP system suitability of Rs≥2.0. Two impurity Peaks are also observed, which further illustrates the resolution capabilities of the analysis. It usually spreads to other parts of the body and Tavda plaquenil small fiber neuropathy is difficult to treat. How to use doxycycline to treat ivermectin farm and fleet skin infections order doxycycline online pharmacy. I also believe incommunicably stromectol in romania that finpecia australia is better than adriamycin as it comes in a liquid form that you can simply swallow and administer straight away. Même si vous vous entendez tous et vous faites gueuler toute la journée, vous ne connaissez évidemment pas le français. Cuando la empresa avanza con este nuevo proceso de prueba, tendrá una cantidad Bobo-Dioulasso ivomec 100ml de medicamentos que no se puede empañar, ya que se trata de una nueva clase de pruebas de salud para la que son necesarios los resultados del esfuerzo humano que ha tomado. This Method illustrates how the MS-compatible HPLC-UV Methods described in various application notes can be successfully adapted for LCMS.

1. Triazolam (internal standard)
2. Alprazolam (API)
3, 4. Impurities

Method Conditions
Column: Cogent Diamond Hydride™, 4µm, 100Å
Catalog No.: 70000-7.5P
Dimensions: 4.6 x 75mm
Mobile Phase:
A: DI Water / 0.1% Formic Acid (v/v)
B: Acetonitrile / 0.1% Formic Acid (v/v)

Time (minutes) %B
0 95
1 95
6 50
7 95

Post Time: 3 minutes
Injection vol.: 1µL
Flow rate: 1.0mL / minute
Detection: UV @ 254nm
Sample Preparation:
Tablet: A 0.25mg strength generic Xanax® tablet was ground and added to a 10mL volumetric flask. After diluting with 50% Solvent A / 50% Solvent B, it was sonicated for 10 minutes. A portion was filtered with a 0.45µm Nylon Syringe Filter (MicroSolv Tech Corp.).
Internal Standard: 1mg / mL Triazolam in Methanol diluent.
Working Solutions: 20µL of the internal standard and 980µL of the tablet extract were mixed. Peak identities were confirmed by individual solutions of the tablet extract and the internal standard.
t0: 0.9 minutes

Note: Alprazolam is a member of the benzo-diazepine class of compounds, prescribed to treat a variety of anxiety-related conditions.


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