Excellent Peak Shape with an Easy to Run Method
This Method is easy to prepare, use and reproduce. Note the Excellent Peak Shape for this Compound. This Methodology will give a long Column life and many Reproducible Runs.
Peak:
Warfarin
Method Conditions
Column: Cogent Bidentate C18™, 4μm, 100Å
Catalog No.: 40018-75P
Dimensions: 4.6 x 75mm
Mobile Phase: 52% Acetonitrile / 48% DI Water / 10mM Sodium Phosphate Monobasic
Temperature: 40°C
Injection vol.: 10μL
Flow rate: 1.5mL / minute
Detection: UV @ 280nm
Notes: Warfarin (also known under the brand names Coumadin, Jantoven, Marevan, and Waran) is an anticoagulant. It is named after the Wisconsin Alumni Research Foundation, which sponsored its development. It was initially marketed as a pesticide against rats and mice, and is still popular for this purpose, although more potent poisons such as Brodifacoum have since been developed.
It is important to note that using Phosphate can alter HPLC Columns and once you use Phosphates with this Column, you should dedicate to Phosphate based Methods.
Attachment
No 81 Warfarin in a Tablet Analyzed with HPLC pdf 0.1 Mb Download File
