Nucleotides Uracil, Guanine, Thymine, Adenine, Excellent Peak Shape and Resolution
This Method is easy to prepare, use and reproduce. Separation is accomplished under 100% Aqueous Conditions yet there is an alternate Selectivity. These bases may be difficult to retain on Columns with ordinary Silica that contain significant amounts of Silanols.
Peaks:
1. Uracil (U)
2. Guanine (G)
3. Thymine (T)
4. Adenine (A)
Method Conditions
Column: Cogent Diamond Hydride™, 4μm, 100Å
Catalog No.: 70000-75P
Dimensions: 4.6 x 75mm
Mobile Phase: DI Water / 0.1% Acetic Acid
Temperature: 25°C
Injection vol.: 2.5μL
Flow rate: 1mL / minute
Detection: UV @ 254nm
Notes: Nucleobases (or Nucleotide Bases) are the parts of DNA and RNA that may be involved in pairing. The main Bases are Cytosine, Guanine, Adenine (DNA and RNA), Thymine (DNA) and Uracil (RNA). They are usually simply called “Bases” in Genetics.
Attachment
Nucleobases Analyzed with HPLC pdf Download File
