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UDP-Glucose, UDP-Galactose and Galactose-1-phosphate from Blood Cells

For most physiologically relevant Nucleotides, use of Mass Spectrometry is the most practical approach for the analysis with biological samples. With Mass Spec, it is essential to Separate UDP-Glucose and UDP-Galactose chromatographically because they are Isomers or Isobaric Compounds, (same molecular weight) and cannot be easily differentiated.

This Method was developed using MS and the samples were blood cell extracts. The Method resulted in excellent Precision.

Peaks:
1. Its chemical Sambalpur ivermectin 500mg name is (3s,4s)-4-fluorobenzyloxy-d-tryptophan. Pillow has a number of benefits like a better blood circulation, lower blood pressure, reduced stress, better immunity and more, and it Masasi plaquenil coupons discounts is safe for the body, however, it may not be appropriate for all the people. Erectile functioning has improved Jenjarum cattle pour on ivermectin since taking this medicine. In a double-blind trial involving 2,037 women undergoing abdominal aortic aneurysm repair, the use of atorvastatin 40 mg showed reductions in death from any cause, ivermectin tablet india manufacturers South Salt Lake myocardial infarction, serious bleeding, and new-onset atrial fibrillation. Read about other uses using liquid ivermectin for dogs preferably of amoxil (category 6) below. UDP-Glucose: Uridine 5′-Diphosphateglucose, 565.0477 m/z (M-H)-
2. UDP-Galactose: Uridine 5′-Diphosphategalactose, 565.0477 m/z (M-H)-
3. Galactose-1-phosphate, 259.0224 m/z (M-H)-

Method Conditions
Column: Cogent Diamond Hydride™ 4μm, 100Å.
Catalog No.: 70000-15P-2
Dimensions: 2.1mm x 150mm
Mobile Phase:
A: DI Water / 0.1% Formic Acid
B: 90% Acetonitrile /10% DI Water / 16.5mM Ammonium Acetate
Gradient:

Time (minutes) %B
0 95
1 95
3 85
6 85
7 75
9 75
10 50
12 50
13 30
15 30
15.01 95

Post Time: 5 minutes
Flow Rate: 0.4mL / minute
Samples Preparation:
Stock Standard Solutions for analysis were prepared in DI Water (1mg / mL of each sample) and were stored at -20°C. For LCMS Analysis samples were diluted 1:100 with 50% Acetonitrile / 50% DI Water Solution.
Detection: ESI – NEG: – Agilent 6210 MSD TOF Mass Spectrometer.

Notes: UDP-Glucose, UDP-Galactose and Galactose 1-phosphate determination can be used for diagnosis of Galactosemia in newborn babies [1-3].

Literature: [1]. Ji-Seon Jeong, Ha-Jeong Kwon, Hye-Ran Yoon, Yong-Moon Lee, Tae-Young Choi, Seon-Pyo Hong, A pulsed amperometric detection method of galactose 1-phosphate for Galactosemia diagnosis, Analytical Biochemistry 376 (2008) 200-2005.
[2]. Ji-Seon Jeong, Hye-Ran Yoon, Seon-Pyo Hong, Development of a new diagnostic method for Galactosemia by high-performance anion exchange chromatography with pulsed amperometric detection, J. Chromatography A, 1140 (2007) 157-162.
[3]. Christopher J. Easley, Lian Ji Jin, Katja B. Presto Elgstoen, Egil Jellum, James P. Landers, Jerome P. Ferrance, Capillary Electrophoresis with Laser-induced Fluorescence Detection for laboratory diagnosis of Galactosemia, J. Chromatography A, 1004 (2003) 29-37.

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